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Student
Name
Mohammed Alnaimi
Student ID 48408
Lesson Chromatography of Food Dyes
Institution Ocean County College
Session 2019L3 CHEM 182 DL1
Course CHEM 182 DL1
Instructor Nancy Marashi
Final Report
Exercise 1
CHEM 182 DL1
Chromatography of Food Dyes
There will be a separation of com ponents of ink. This could create an error with the com ponents
that are trying to separate. This said, a pen could not be used.
1. What might happen if a student used a pen to mark the baseline on the
chromatography paper?
The solution will m ove to the top and you would not be able to calculate the Rf value that is
com pared to the distance that a com ponent traveled in the stationary phase reached by the
solvent.
2. What would happen if the student did not stop the development before the solvent
reached the top of the paper?
The solvent would wash away the dye.
3. What would happen if the solvent level was above that of the dye spots on the paper
at the start of the development?
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Photo 1: Chromatogram
Data Table 1: Chromatogram Data
Because the solvents used for chrom atography are volatile. If it is not m arked im m ediately,
there
is a possibility that it m ay evaporate and then you would not be able to draw the solvent.
4. Why is it important to mark the solvent level on the chromatography paper as soon as
you remove it f rom the Petri dish?
Sample Solvent front (mm)
Blue 1
Blue 2
Red 3
Red 40
Yellow 5
Yellow 6
UK
48
48
48
48
48
48
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Sample Spot 1 mm
Blue 1
Blue 2
Red 3
Red 40
Yellow 5
Yellow 6
UK
Sample Spot 1 R
Blue 1
Blue 2
Red 3
Red 40
Yellow 5
Yellow 6
UK
Sample Spot 2 mm
Blue 1
Blue 2
48
32
0
0
1
2
3
1
f
1.5
0
0
48
24
16
48
40
1
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Red 3
Red 40
Yellow 5
Yellow 6
UK
Sample Spot 2 R
Blue 1
Blue 2
Red 3
Red 40
Yellow 5
Yellow 6
UK
Sample Spot 3 mm
Blue 1
Blue 2
Red 3
Red 40
Yellow 5
Yellow 6
-4
3
10
7
3
f
1.2
48
-12
16
4.8
6.86
16
47
2
-6
3
18
8
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Data Table 2: FD&C Dyes in Sample
UK
Sample Spot 3 R
Blue 1
Blue 2
Red 3
Red 40
Yellow 5
Yellow 6
UK
4
f
1.02
-24
-8
16
2.67
6
12
Sample: FD&C Dyes in Sample
Unknown Sample Red 40
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